PlantImRecepV1, Main, Exploration, bibRecord, 000177

Distinct anti-proliferative effects of herbal melanin on human acute monocytic leukemia THP-1 cells and embryonic kidney HEK293 cells.

Identifieur interne : 000177 ( Main/Exploration ); précédent : 000176; suivant : 000178

Distinct anti-proliferative effects of herbal melanin on human acute monocytic leukemia THP-1 cells and embryonic kidney HEK293 cells.

Auteurs : Adila El-Obeid [Arabie saoudite, Soudan] ; Hala Alajmi [Arabie saoudite] ; Mashael Harbi [Arabie saoudite] ; Wesam Bin Yahya [Arabie saoudite] ; Hamad Al-Eidi [Arabie saoudite] ; Monira Alaujan [Arabie saoudite] ; Adil Haseeb [Arabie saoudite] ; Thadeo Trivilegio [Arabie saoudite] ; Alshaimaa Alhallaj [Arabie saoudite] ; Saleh Alghamdi [Arabie saoudite] ; Abdul-Wali Ajlouni [Arabie saoudite] ; Sabine Matou-Nasri [Arabie saoudite]

Source :

BMC complementary medicine and therapies [ 2662-7671 ] ; 2020.

RBID : pubmed:32448225

Descripteurs français

KwdFr :
- Apoptose (effets des médicaments et des substances chimiques), Cellules HEK293 (MeSH), Cellules THP-1 (MeSH), Extraits de plantes (pharmacologie), Graines (composition chimique), Humains (MeSH), Leucémie aigüe monoblastique (anatomopathologie), Leucémie aigüe monoblastique (traitement médicamenteux), Mélanines (pharmacologie), Nigella sativa (composition chimique), Points de contrôle du cycle cellulaire (effets des médicaments et des substances chimiques), Relation dose-effet des médicaments (MeSH), Récepteur de type Toll-4 (métabolisme).
MESH :
- anatomopathologie : Leucémie aigüe monoblastique.
- composition chimique : Graines, Nigella sativa.
- effets des médicaments et des substances chimiques : Apoptose, Points de contrôle du cycle cellulaire.
- métabolisme : Récepteur de type Toll-4.
- pharmacologie : Extraits de plantes, Mélanines.
- traitement médicamenteux : Leucémie aigüe monoblastique.
- Cellules HEK293, Cellules THP-1, Humains, Relation dose-effet des médicaments.

English descriptors

KwdEn :
- Apoptosis (drug effects), Cell Cycle Checkpoints (drug effects), Dose-Response Relationship, Drug (MeSH), HEK293 Cells (MeSH), Humans (MeSH), Leukemia, Monocytic, Acute (drug therapy), Leukemia, Monocytic, Acute (pathology), Melanins (pharmacology), Nigella sativa (chemistry), Plant Extracts (pharmacology), Seeds (chemistry), THP-1 Cells (MeSH), Toll-Like Receptor 4 (metabolism).
MESH :
- chemical , metabolism : Toll-Like Receptor 4.
- chemical , pharmacology : Melanins, Plant Extracts.
- chemistry : Nigella sativa, Seeds.
- drug effects : Apoptosis, Cell Cycle Checkpoints.
- drug therapy : Leukemia, Monocytic, Acute.
- pathology : Leukemia, Monocytic, Acute.
- Dose-Response Relationship, Drug, HEK293 Cells, Humans, THP-1 Cells.

Abstract

BACKGROUND

Herbal melanin (HM) is a dark pigment extracted from the seed coat of Nigella sativa L. and known to exert biological effects via toll-like receptor 4 (TLR4). Recently, TLR4 was described as involved in natural programmed cell death (apoptosis). Tumor and embryonic cells are used as in vitro cellular models for drug and anti-cancer agent screening. To date, no cytotoxic studies have been reported of HM in TLR4-positive acute monocytic leukemia THP-1 cells compared to TLR4-negative human embryonic kidney HEK293 cells.

METHODS

We studied the anti-proliferative effects of several HM concentrations on THP-1 and HEK293 cells by evaluating cell viability using the CellTiter-Glo® luminescent assay, assessing the TLR4 expression level, determining the apoptotic status, and analyzing the cell cycle distribution using flow cytometry. Apoptotic pathways were investigated using mitochondrial transition pore opening, caspase activity assays and immunoblot technology.

RESULTS

Low HM concentrations did not affect THP-1 cell viability, but high HM concentrations (62.5-500 μg/mL) did decrease THP-1 cell viability and induced G

CONCLUSION

HM exerts distinct anti-proliferative effects on human acute monocytic leukemia and embryonic kidney cells mainly through cell cycle interference in a TLR4-independent manner and through apoptosis induction in a TLR4-dependent manner, as observed in only the THP-1 cells.

DOI: 10.1186/s12906-020-02944-1
PubMed: 32448225
PubMed Central: PMC7245827

Affiliations:

Arabie saoudite, Soudan

Links toward previous steps (curation, corpus...)

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Le document en format XML

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<author><name sortKey="Trivilegio, Thadeo" sort="Trivilegio, Thadeo" uniqKey="Trivilegio T" first="Thadeo" last="Trivilegio">Thadeo Trivilegio</name>
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<author><name sortKey="Alhallaj, Alshaimaa" sort="Alhallaj, Alshaimaa" uniqKey="Alhallaj A" first="Alshaimaa" last="Alhallaj">Alshaimaa Alhallaj</name>
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<author><name sortKey="Alghamdi, Saleh" sort="Alghamdi, Saleh" uniqKey="Alghamdi S" first="Saleh" last="Alghamdi">Saleh Alghamdi</name>
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<country xml:lang="fr">Arabie saoudite</country>
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<author><name sortKey="Ajlouni, Abdul Wali" sort="Ajlouni, Abdul Wali" uniqKey="Ajlouni A" first="Abdul-Wali" last="Ajlouni">Abdul-Wali Ajlouni</name>
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<author><name sortKey="Matou Nasri, Sabine" sort="Matou Nasri, Sabine" uniqKey="Matou Nasri S" first="Sabine" last="Matou-Nasri">Sabine Matou-Nasri</name>
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<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426</wicri:regionArea>
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<affiliation wicri:level="1"><nlm:affiliation>King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia. matouepnasrisa@ngha.med.sa.</nlm:affiliation>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">Distinct anti-proliferative effects of herbal melanin on human acute monocytic leukemia THP-1 cells and embryonic kidney HEK293 cells.</title>
<author><name sortKey="El Obeid, Adila" sort="El Obeid, Adila" uniqKey="El Obeid A" first="Adila" last="El-Obeid">Adila El-Obeid</name>
<affiliation wicri:level="1"><nlm:affiliation>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426, Saudi Arabia.</nlm:affiliation>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426</wicri:regionArea>
<wicri:noRegion>11426</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Department of Biobank, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, Riyadh, Saudi Arabia.</nlm:affiliation>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Department of Biobank, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>School of Pharmacy, Ahfad University for Women, Khartoum, Sudan.</nlm:affiliation>
<country xml:lang="fr">Soudan</country>
<wicri:regionArea>School of Pharmacy, Ahfad University for Women, Khartoum</wicri:regionArea>
<wicri:noRegion>Khartoum</wicri:noRegion>
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<affiliation wicri:level="1"><nlm:affiliation>King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.</nlm:affiliation>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>King Saud bin Abdulaziz University for Health Sciences, Riyadh</wicri:regionArea>
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<author><name sortKey="Alajmi, Hala" sort="Alajmi, Hala" uniqKey="Alajmi H" first="Hala" last="Alajmi">Hala Alajmi</name>
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<author><name sortKey="Harbi, Mashael" sort="Harbi, Mashael" uniqKey="Harbi M" first="Mashael" last="Harbi">Mashael Harbi</name>
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<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426</wicri:regionArea>
<wicri:noRegion>11426</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.</nlm:affiliation>
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<author><name sortKey="Yahya, Wesam Bin" sort="Yahya, Wesam Bin" uniqKey="Yahya W" first="Wesam Bin" last="Yahya">Wesam Bin Yahya</name>
<affiliation wicri:level="1"><nlm:affiliation>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426, Saudi Arabia.</nlm:affiliation>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426</wicri:regionArea>
<wicri:noRegion>11426</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.</nlm:affiliation>
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<author><name sortKey="Al Eidi, Hamad" sort="Al Eidi, Hamad" uniqKey="Al Eidi H" first="Hamad" last="Al-Eidi">Hamad Al-Eidi</name>
<affiliation wicri:level="1"><nlm:affiliation>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426, Saudi Arabia.</nlm:affiliation>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426</wicri:regionArea>
<wicri:noRegion>11426</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.</nlm:affiliation>
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<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
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<author><name sortKey="Alaujan, Monira" sort="Alaujan, Monira" uniqKey="Alaujan M" first="Monira" last="Alaujan">Monira Alaujan</name>
<affiliation wicri:level="1"><nlm:affiliation>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426, Saudi Arabia.</nlm:affiliation>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426</wicri:regionArea>
<wicri:noRegion>11426</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.</nlm:affiliation>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>King Saud bin Abdulaziz University for Health Sciences, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Haseeb, Adil" sort="Haseeb, Adil" uniqKey="Haseeb A" first="Adil" last="Haseeb">Adil Haseeb</name>
<affiliation wicri:level="1"><nlm:affiliation>Attosecond-Laser Laboratory, Faculty of Science, Kind Saud University, Riyadh, Saudi Arabia.</nlm:affiliation>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Attosecond-Laser Laboratory, Faculty of Science, Kind Saud University, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Trivilegio, Thadeo" sort="Trivilegio, Thadeo" uniqKey="Trivilegio T" first="Thadeo" last="Trivilegio">Thadeo Trivilegio</name>
<affiliation wicri:level="1"><nlm:affiliation>King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.</nlm:affiliation>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>King Saud bin Abdulaziz University for Health Sciences, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Core Facility, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, Riyadh, Saudi Arabia.</nlm:affiliation>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Core Facility, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Alhallaj, Alshaimaa" sort="Alhallaj, Alshaimaa" uniqKey="Alhallaj A" first="Alshaimaa" last="Alhallaj">Alshaimaa Alhallaj</name>
<affiliation wicri:level="1"><nlm:affiliation>King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.</nlm:affiliation>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>King Saud bin Abdulaziz University for Health Sciences, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>Core Facility, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, Riyadh, Saudi Arabia.</nlm:affiliation>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Core Facility, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Alghamdi, Saleh" sort="Alghamdi, Saleh" uniqKey="Alghamdi S" first="Saleh" last="Alghamdi">Saleh Alghamdi</name>
<affiliation wicri:level="1"><nlm:affiliation>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426, Saudi Arabia.</nlm:affiliation>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426</wicri:regionArea>
<wicri:noRegion>11426</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.</nlm:affiliation>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>King Saud bin Abdulaziz University for Health Sciences, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Ajlouni, Abdul Wali" sort="Ajlouni, Abdul Wali" uniqKey="Ajlouni A" first="Abdul-Wali" last="Ajlouni">Abdul-Wali Ajlouni</name>
<affiliation wicri:level="1"><nlm:affiliation>Toxicology Department, Naif Arab University for Security Sciences, Riyadh, Saudi Arabia.</nlm:affiliation>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Toxicology Department, Naif Arab University for Security Sciences, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Matou Nasri, Sabine" sort="Matou Nasri, Sabine" uniqKey="Matou Nasri S" first="Sabine" last="Matou-Nasri">Sabine Matou-Nasri</name>
<affiliation wicri:level="1"><nlm:affiliation>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426, Saudi Arabia. matouepnasrisa@ngha.med.sa.</nlm:affiliation>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426</wicri:regionArea>
<wicri:noRegion>11426</wicri:noRegion>
</affiliation>
<affiliation wicri:level="1"><nlm:affiliation>King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia. matouepnasrisa@ngha.med.sa.</nlm:affiliation>
<country xml:lang="fr">Arabie saoudite</country>
<wicri:regionArea>King Saud bin Abdulaziz University for Health Sciences, Riyadh</wicri:regionArea>
<wicri:noRegion>Riyadh</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j">BMC complementary medicine and therapies</title>
<idno type="eISSN">2662-7671</idno>
<imprint><date when="2020" type="published">2020</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Apoptosis (drug effects)</term>
<term>Cell Cycle Checkpoints (drug effects)</term>
<term>Dose-Response Relationship, Drug (MeSH)</term>
<term>HEK293 Cells (MeSH)</term>
<term>Humans (MeSH)</term>
<term>Leukemia, Monocytic, Acute (drug therapy)</term>
<term>Leukemia, Monocytic, Acute (pathology)</term>
<term>Melanins (pharmacology)</term>
<term>Nigella sativa (chemistry)</term>
<term>Plant Extracts (pharmacology)</term>
<term>Seeds (chemistry)</term>
<term>THP-1 Cells (MeSH)</term>
<term>Toll-Like Receptor 4 (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Apoptose (effets des médicaments et des substances chimiques)</term>
<term>Cellules HEK293 (MeSH)</term>
<term>Cellules THP-1 (MeSH)</term>
<term>Extraits de plantes (pharmacologie)</term>
<term>Graines (composition chimique)</term>
<term>Humains (MeSH)</term>
<term>Leucémie aigüe monoblastique (anatomopathologie)</term>
<term>Leucémie aigüe monoblastique (traitement médicamenteux)</term>
<term>Mélanines (pharmacologie)</term>
<term>Nigella sativa (composition chimique)</term>
<term>Points de contrôle du cycle cellulaire (effets des médicaments et des substances chimiques)</term>
<term>Relation dose-effet des médicaments (MeSH)</term>
<term>Récepteur de type Toll-4 (métabolisme)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Toll-Like Receptor 4</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Melanins</term>
<term>Plant Extracts</term>
</keywords>
<keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Leucémie aigüe monoblastique</term>
</keywords>
<keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Nigella sativa</term>
<term>Seeds</term>
</keywords>
<keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Graines</term>
<term>Nigella sativa</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Apoptosis</term>
<term>Cell Cycle Checkpoints</term>
</keywords>
<keywords scheme="MESH" qualifier="drug therapy" xml:lang="en"><term>Leukemia, Monocytic, Acute</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Apoptose</term>
<term>Points de contrôle du cycle cellulaire</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Récepteur de type Toll-4</term>
</keywords>
<keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Leukemia, Monocytic, Acute</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Extraits de plantes</term>
<term>Mélanines</term>
</keywords>
<keywords scheme="MESH" qualifier="traitement médicamenteux" xml:lang="fr"><term>Leucémie aigüe monoblastique</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Dose-Response Relationship, Drug</term>
<term>HEK293 Cells</term>
<term>Humans</term>
<term>THP-1 Cells</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Cellules HEK293</term>
<term>Cellules THP-1</term>
<term>Humains</term>
<term>Relation dose-effet des médicaments</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p><b>BACKGROUND</b>
</p>
<p>Herbal melanin (HM) is a dark pigment extracted from the seed coat of Nigella sativa L. and known to exert biological effects via toll-like receptor 4 (TLR4). Recently, TLR4 was described as involved in natural programmed cell death (apoptosis). Tumor and embryonic cells are used as in vitro cellular models for drug and anti-cancer agent screening. To date, no cytotoxic studies have been reported of HM in TLR4-positive acute monocytic leukemia THP-1 cells compared to TLR4-negative human embryonic kidney HEK293 cells.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>METHODS</b>
</p>
<p>We studied the anti-proliferative effects of several HM concentrations on THP-1 and HEK293 cells by evaluating cell viability using the CellTiter-Glo® luminescent assay, assessing the TLR4 expression level, determining the apoptotic status, and analyzing the cell cycle distribution using flow cytometry. Apoptotic pathways were investigated using mitochondrial transition pore opening, caspase activity assays and immunoblot technology.</p>
</div>
<div type="abstract" xml:lang="en"><p><b>RESULTS</b>
</p>
<p>Low HM concentrations did not affect THP-1 cell viability, but high HM concentrations (62.5-500 μg/mL) did decrease THP-1 cell viability and induced G</p>
</div>
<div type="abstract" xml:lang="en"><p><b>CONCLUSION</b>
</p>
<p>HM exerts distinct anti-proliferative effects on human acute monocytic leukemia and embryonic kidney cells mainly through cell cycle interference in a TLR4-independent manner and through apoptosis induction in a TLR4-dependent manner, as observed in only the THP-1 cells.</p>
</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">32448225</PMID>
<DateCompleted><Year>2020</Year>
<Month>11</Month>
<Day>02</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>11</Month>
<Day>02</Day>
</DateRevised>
<Article PubModel="Electronic"><Journal><ISSN IssnType="Electronic">2662-7671</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>20</Volume>
<Issue>1</Issue>
<PubDate><Year>2020</Year>
<Month>May</Month>
<Day>24</Day>
</PubDate>
</JournalIssue>
<Title>BMC complementary medicine and therapies</Title>
<ISOAbbreviation>BMC Complement Med Ther</ISOAbbreviation>
</Journal>
<ArticleTitle>Distinct anti-proliferative effects of herbal melanin on human acute monocytic leukemia THP-1 cells and embryonic kidney HEK293 cells.</ArticleTitle>
<Pagination><MedlinePgn>154</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.1186/s12906-020-02944-1</ELocationID>
<Abstract><AbstractText Label="BACKGROUND" NlmCategory="BACKGROUND">Herbal melanin (HM) is a dark pigment extracted from the seed coat of Nigella sativa L. and known to exert biological effects via toll-like receptor 4 (TLR4). Recently, TLR4 was described as involved in natural programmed cell death (apoptosis). Tumor and embryonic cells are used as in vitro cellular models for drug and anti-cancer agent screening. To date, no cytotoxic studies have been reported of HM in TLR4-positive acute monocytic leukemia THP-1 cells compared to TLR4-negative human embryonic kidney HEK293 cells.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">We studied the anti-proliferative effects of several HM concentrations on THP-1 and HEK293 cells by evaluating cell viability using the CellTiter-Glo® luminescent assay, assessing the TLR4 expression level, determining the apoptotic status, and analyzing the cell cycle distribution using flow cytometry. Apoptotic pathways were investigated using mitochondrial transition pore opening, caspase activity assays and immunoblot technology.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">Low HM concentrations did not affect THP-1 cell viability, but high HM concentrations (62.5-500 μg/mL) did decrease THP-1 cell viability and induced G<sub>0</sub>
/G<sub>1</sub>
 phase cell cycle arrest. Only at the highest concentration (500 μg/mL), HM slightly increased the TLR4 expression on the THP-1 cell surface, concomitantly upregulated TLR4 whole protein and gene expression, and induced apoptosis in THP-1 cells via activation of the extrinsic and intrinsic pathways. No change of apoptotic status was noticed in TLR4-negative HEK293 cells, although HM decreased HEK293 cell viability and induced cell growth arrest in the G<sub>2</sub>
 phase.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">HM exerts distinct anti-proliferative effects on human acute monocytic leukemia and embryonic kidney cells mainly through cell cycle interference in a TLR4-independent manner and through apoptosis induction in a TLR4-dependent manner, as observed in only the THP-1 cells.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>El-Obeid</LastName>
<ForeName>Adila</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426, Saudi Arabia.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Department of Biobank, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>School of Pharmacy, Ahfad University for Women, Khartoum, Sudan.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Alajmi</LastName>
<ForeName>Hala</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>Department of Biobank, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Harbi</LastName>
<ForeName>Mashael</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426, Saudi Arabia.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yahya</LastName>
<ForeName>Wesam Bin</ForeName>
<Initials>WB</Initials>
<AffiliationInfo><Affiliation>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426, Saudi Arabia.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Al-Eidi</LastName>
<ForeName>Hamad</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426, Saudi Arabia.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Alaujan</LastName>
<ForeName>Monira</ForeName>
<Initials>M</Initials>
<AffiliationInfo><Affiliation>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426, Saudi Arabia.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Haseeb</LastName>
<ForeName>Adil</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>Attosecond-Laser Laboratory, Faculty of Science, Kind Saud University, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Trivilegio</LastName>
<ForeName>Thadeo</ForeName>
<Initials>T</Initials>
<AffiliationInfo><Affiliation>King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Core Facility, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Alhallaj</LastName>
<ForeName>Alshaimaa</ForeName>
<Initials>A</Initials>
<AffiliationInfo><Affiliation>King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>Core Facility, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Alghamdi</LastName>
<ForeName>Saleh</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426, Saudi Arabia.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ajlouni</LastName>
<ForeName>Abdul-Wali</ForeName>
<Initials>AW</Initials>
<AffiliationInfo><Affiliation>Toxicology Department, Naif Arab University for Security Sciences, Riyadh, Saudi Arabia.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Matou-Nasri</LastName>
<ForeName>Sabine</ForeName>
<Initials>S</Initials>
<Identifier Source="ORCID">http://orcid.org/0000-0003-4372-2903</Identifier>
<AffiliationInfo><Affiliation>Cell and Gene Therapy Group, Medical Genomics Research Department, King Abdullah International Medical Research Center, Ministry of National Guard Health Affairs, P.O. Box 22490, Riyadh, 11426, Saudi Arabia. matouepnasrisa@ngha.med.sa.</Affiliation>
</AffiliationInfo>
<AffiliationInfo><Affiliation>King Saud bin Abdulaziz University for Health Sciences, Riyadh, Saudi Arabia. matouepnasrisa@ngha.med.sa.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<GrantList CompleteYN="Y"><Grant><GrantID>RC15/106</GrantID>
<Agency>King Abdullah International Medical Research Center</Agency>
<Country></Country>
</Grant>
</GrantList>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2020</Year>
<Month>05</Month>
<Day>24</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>BMC Complement Med Ther</MedlineTA>
<NlmUniqueID>101761232</NlmUniqueID>
<ISSNLinking>2662-7671</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D008543">Melanins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010936">Plant Extracts</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C495345">TLR4 protein, human</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D051197">Toll-Like Receptor 4</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D017209" MajorTopicYN="N">Apoptosis</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D059447" MajorTopicYN="N">Cell Cycle Checkpoints</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004305" MajorTopicYN="N">Dose-Response Relationship, Drug</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D057809" MajorTopicYN="N">HEK293 Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D007948" MajorTopicYN="N">Leukemia, Monocytic, Acute</DescriptorName>
<QualifierName UI="Q000188" MajorTopicYN="N">drug therapy</QualifierName>
<QualifierName UI="Q000473" MajorTopicYN="Y">pathology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008543" MajorTopicYN="N">Melanins</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D031881" MajorTopicYN="N">Nigella sativa</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010936" MajorTopicYN="N">Plant Extracts</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012639" MajorTopicYN="N">Seeds</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000074084" MajorTopicYN="N">THP-1 Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D051197" MajorTopicYN="N">Toll-Like Receptor 4</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
<KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Apoptosis</Keyword>
<Keyword MajorTopicYN="N">Cell cycle</Keyword>
<Keyword MajorTopicYN="N">HEK293</Keyword>
<Keyword MajorTopicYN="N">Herbal melanin</Keyword>
<Keyword MajorTopicYN="N">Human embryonic kidney</Keyword>
<Keyword MajorTopicYN="N">THP-1</Keyword>
<Keyword MajorTopicYN="N">Toll-like receptor 4</Keyword>
</KeywordList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2019</Year>
<Month>07</Month>
<Day>23</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2020</Year>
<Month>05</Month>
<Day>07</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2020</Year>
<Month>5</Month>
<Day>26</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
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<Month>5</Month>
<Day>26</Day>
<Hour>6</Hour>
<Minute>0</Minute>
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<PubMedPubDate PubStatus="medline"><Year>2020</Year>
<Month>11</Month>
<Day>3</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
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<ReferenceList><Reference><Citation>Biochim Biophys Acta Mol Basis Dis. 2017 Jan;1863(1):239-252</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27641442</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Sci Immunol. 2017 Nov 10;2(17):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29127106</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cells Tissues Organs. 2018;205(1):1-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29393161</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Acta Haematol. 2014;132(1):5-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24356089</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Nanomedicine. 2018 Sep 06;13:5207-5213</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30233181</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Mol Sci. 2018 Jan 16;19(1):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29337925</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Basic Clin Pharmacol Toxicol. 2017 Jun;120(6):515-522</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28027430</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Front Immunol. 2017 Dec 18;8:1820</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29326706</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2017 Jun 9;356(6342):1064-1068</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28596363</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Mol Sci. 2018 Sep 12;19(9):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30213077</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Asian Pac J Trop Biomed. 2013 May;3(5):337-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23646296</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Semin Cancer Biol. 2015 Dec;35 Suppl:S151-S184</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25951989</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Med Rep. 2016 Nov;14(5):4505-4510</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27748869</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nutr Res Pract. 2017 Jun;11(3):190-197</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28584575</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Clin Cancer Res. 2019 Feb 15;25(4):1185-1195</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30093453</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Immunol. 2003 Oct 15;171(8):4294-303</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14530354</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mar Drugs. 2019 Jan 23;17(2):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">30678065</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Sci Rep. 2017 Feb 14;7:42129</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28195138</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Med. 1990 Mar 1;171(3):629-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2307930</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biomedicines. 2019 Jun 03;7(2):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">31163699</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Evid Based Complement Alternat Med. 2015;2015:682019</Citation>
<ArticleIdList><ArticleId IdType="pubmed">25810745</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biomacromolecules. 2017 Jun 12;18(6):1908-1917</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28510430</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Mol Cancer Res. 2018 May;16(5):833-845</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29523762</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Int J Mol Sci. 2018 Feb 02;19(2):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29393886</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Sci Rep. 2017 Mar 23;7:44990</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28332554</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Res. 2018 Jan;28(1):9-21</Citation>
<ArticleIdList><ArticleId IdType="pubmed">29076500</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Neurochem. 2008 Jun 1;105(6):2489-500</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18399961</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Med Mycol. 2014 Jul;52(5):445-54</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24934806</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur Respir J. 2017 May 23;49(5):</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28536249</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Trends Parasitol. 2005 Apr;21(4):192-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15780842</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Immunobiology. 2017 Feb;222(2):198-205</Citation>
<ArticleIdList><ArticleId IdType="pubmed">27720227</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cancer Gene Ther. 2002 Aug;9(8):673-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12136428</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Phytomedicine. 2006 May;13(5):324-33</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16635740</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Phytomedicine. 2009 May;16(5):477-84</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19103478</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Sci Rep. 2017 Mar 08;7:43834</Citation>
<ArticleIdList><ArticleId IdType="pubmed">28272417</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Med Chem. 2020;16(3):307-325</Citation>
<ArticleIdList><ArticleId IdType="pubmed">31241436</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Cell Biol Int. 2003;27(2):135-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12662970</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Arabie saoudite</li>
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	Serveur d'exploration sur les récepteurs immunitaires végétaux
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Serveur d'exploration sur les récepteurs immunitaires végétaux

Distinct anti-proliferative effects of herbal melanin on human acute monocytic leukemia THP-1 cells and embryonic kidney HEK293 cells.

Distinct anti-proliferative effects of herbal melanin on human acute monocytic leukemia THP-1 cells and embryonic kidney HEK293 cells.

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